2 d preparative comb Search Results


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FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
Ve Cadherin Primary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
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FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
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Vector Laboratories fluorescein conjugated ulex europaeus agglutinin 1 uea 1
FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
Fluorescein Conjugated Ulex Europaeus Agglutinin 1 Uea 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti mouse tgfβ 3 blocking antibody
FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
Anti Mouse Tgfβ 3 Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
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Cambridge Isotope Laboratories 13 c 6 d-glucose
FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
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FIG. 4. ERG activation preserves <t>VE-cadherin</t> in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated <t>with</t> <t>VEGF</t> shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
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Image Search Results


FIG. 4. ERG activation preserves VE-cadherin in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated with VEGF shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).

Journal: Shock

Article Title: ETS-Related Gene Activation Preserves Adherens Junctions and Permeability in Microvascular Endothelial Cells

doi: 10.1097/shk.0000000000001899

Figure Lengend Snippet: FIG. 4. ERG activation preserves VE-cadherin in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated with VEGF shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).

Article Snippet: VEGF was purchased from R&D Systems (Minneapolis, MN). b-Catenin and VE-cadherin primary antibodies and FITC-tagged secondary antibodies were obtained from Santa Cruz.

Techniques: Activation Assay, Transfection, CRISPR, Knockdown, Plasmid Preparation, Disruption, Control