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Image Search Results
Journal: Shock
Article Title: ETS-Related Gene Activation Preserves Adherens Junctions and Permeability in Microvascular Endothelial Cells
doi: 10.1097/shk.0000000000001899
Figure Lengend Snippet: FIG. 4. ERG activation preserves VE-cadherin in endothelial adherens junctions in human lung microvascular endothelial cells. The group transfected with ERG CRISPR/Cas9 knockdown plasmid and the group treated with VEGF shows disruption of the adherens junction proteins VE-cadherin evidenced by discontinuity of their localization at the cell–cell junctions compared to their respective control groups (60). The group transfected with ERG CRISPR activation plasmid followed by VEGF treatment, shows relatively intact adherens junctions, evidenced by their continuous localization at the cell-cell junctions compared with VEGF alone group. Arrows indicate areas of cell-cell contacts where VE-cadherin is expected normally but not localized abundantly compared to other groups (n ¼ 4).
Article Snippet: VEGF was purchased from R&D Systems (Minneapolis, MN). b-Catenin and
Techniques: Activation Assay, Transfection, CRISPR, Knockdown, Plasmid Preparation, Disruption, Control